Investigation of some structural-functional peculiarities of the immunogenic fragment Ki-67 antigen. Generation of polyclonal and monoclonal antibodies to Ki-67
نویسندگان
چکیده
منابع مشابه
Comparison of Ki-67 equivalent antibodies.
AIMS To compare commercially available Ki-67 equivalent antibodies with regard to qualitative and quantitative immunohistochemical staining characteristics. METHODS The following antibodies were used: monoclonal MIB-1 (Immunotech), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (Dako). All immunostainings were evaluated in squamous epithelium from fo...
متن کاملKi - 67 contributes to normal
Department of Molecular, Cell and Cancer Biology 7 University of Massachusetts Medical School, Worcester, MA 01605, USA 8 9 Program in Bioinformatics and Integrative Biology 10 University of Massachusetts Medical School, Worcester, MA 01605, USA. 11 12 Program in Molecular Medicine 13 University of Massachusetts Medical School, Worcester, MA 01605, USA. 14 15 16 Running title: Ki-67 affects S p...
متن کاملProliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies
BACKGROUND Ki67 labeling index (Ki67 LI), the percentage Ki67 immunoreactive cells, is a measure of tumor proliferation, with important clinical relevance in breast cancer, and it is extremely important to standardize its evaluation. AIM To test the efficacy of computer assisted image analysis (CAIA) applied to completely digitized slides and to assess its feasibility in routine practice and ...
متن کاملAssessment of cell proliferation in hydatidiform mole using monoclonal antibody MIB1 to Ki-67 antigen.
AIMS To assess the role of Ki67 immunoreactivity in predicting the clinical progress of hydatidiform mole. METHODS Tissue from 87 hydatidiform moles, 11 normal first trimester placentas, 11 normal term placentas and 17 spontaneous abortions were examined for expression of Ki67 antigen, using the monoclonal antibody MIB1. RESULTS Ki67 immunoreactivity was significantly higher in the tissue f...
متن کاملEpitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein).
AIMS--To elucidate the fine specificities of the antibodies MIB 1 and MIB 3 and of additional monoclonal antibodies which also recognise the Ki-67 protein (MIB 5, IND.64, JG-67-2a). METHODS--Different parts of the Ki-67 protein cDNA were expressed in Escherichia coli. Bacterial lysates were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted on to nitr...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 2005
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0006ea